Skip to content

BIOLOGICAL CHEMISTRY

Course info

  • Course: BIOLOGICAL CHEMISTRY
  • Year: Second year
  • Semester: Second semester
  • Activity type: Basic educational activities
  • CFU: 9
  • SSD: BIO/10

Characteristics

TitleCodeCFUSSDSubject areaLecture (Hours)Exercise (Hours)Lab (Hours)
Attività formative di baseA9BIO/10Discipline biologiche64N.D.12

Channels

NESSUNA CANALIZZAZIONE
ProfessorDepartment
MARTINO LUIGI DI SALVO

Objectives

The aim of the course is the understanding of the biochemical and molecular basis of complex biological systems. This will be pursued through: i) description of the principal features and reactions of the most important biomolecules and biological macromolecules (carbohydrates, lipids, amino acids, proteins, nucleotides); ii) study of biological membranes, principal transport systems, bioenergetics and metabolism; ii) study of structure-function relationship in proteins, molecular evolution of proteins, enzymology; iv) description of main catabolic and anabolic pathways involving carbohydrates, lipids and amino acids. Moreover, biochemical methodologies will be taken into consideration by developing experimental projects.

Textbooks

Nelson, Cox - Lehninger Principles of Biochemistry, 4th edition
Jeremy M. Berg, John L. Tymoczko, Lubert Stryer – Biochemistry, 5th edition
Garret, Grisham - Principles of Biochemistry With a Human Focus, 2nd edition
Donald Voet, Charlotte W. Pratt, Judith G. Voet - Principles of Biochemistry, 4th EditionBiochemistry: Concepts and Connections Plus MasteringChemistry with eText -- Access Card Package
Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews - Biochemistry Concepts and Connections

Programme

Lezioni teoriche:· Le biomolecole. L'evoluzione prebiotica.· L'acqua: i suoi effetti sulle biomolecole.· Aminoacidi e peptidi.· Le proteine.· Gli enzimi.· I lipidi.· Membrane biologiche, trasporto di membrana.· I carboidrati.· Nucleotidi e acidi nucleici.· Principi di bioenergetica.· Glicolisi e catabolismo degli zuccheri.· Biosintesi dei carboidrati.· Regolazione del metabolismo dei carboidrati.· Ciclo dell'acido citrico e regolazione.· Fosforilazione ossidativa.· Ossidazione degli acidi grassi.· Metabolismo degli aminoacidi e ciclo dell’urea.· Cenni sulla biosintesi dei lipidi e dei nucleotidi.· Meccanismi molecolari di trasduzione del segnale.Esercitazioni in laboratorio:· Modelli molecolari· Preparazione di estratti cellulari e dosaggio proteico· Cromatografia di proteine· Elettroforesi su gel di poliacrilammide· Cinetica enzimatica.