Biomedical Laboratory techniques

Nucleic acids biochemistry: purines and pyrimidines, nucleosides, nucleotides. DNA and RNA structure, DNA conformation and topology. DNA replication in eukaryotes and procaryotes. DNA repair mechanisms. DNA transcription and RNA processing. RNA translation and protein synthesis. Regulation of gene expression. Post-translational modifications. Epigenetic modifications. Nucleic acids isolation and purification. Basic techniques: nucleic acids electrophoresis, cloning, restriction enzymes, hybridization (Northern and Southern blotting, microarray, microchip). Capillary electrophoresis. Polymerase Chain Reaction (PCR). Endpoint PCR, real-Time PCR, digital-PCR. Gene expression analysis. Nucleic acids sequencing Sanger sequencing, Pyrosequencing, RNA-Seq, ATAC-Seq. Genome wide analyses. Genome alteration (mutation analysis, dieci and inverse hybridization, GWAS, MLPA). New biotechnology products, genome editing, CRISPR/Cas9. DNA-proteins interactions: DNA footprint, ChIP. Techniques for studying epigenetic modifications: DNA methylation, microRNA espressione, histone modifications.

Basic knowledge of cellular and molecular biology and biochemistry

Francesco Amaldi, Piero Benedetti, Graziano Pesole, Paolo Plevani TECNICHE E METODI PER LA BIOLOGIA MOLECOLARE Ed.: CEA/Zanichelli

Frontal lessons, flipped classroom, discussion of practical issues Remote lessons if necessary due to Covid-19 restrictions. Laboratory experiences (if possible due to Covid-19 restrictions).

Presence in classroom or connection by Zoom/Meet platform

Oral exams. Possible written test at the end of the course. Self-evaluation anonym (online tests) during the course.

Frontal lessons, flipped classroom, discussion of practical issues Remote lessons if necessary due to Covid-19 restrictions. Laboratory experiences (if possible due to Covid-19 restrictions).