Nursing

USE OF BIOCHEMICAL DATA IN CLINICAL MEDICINE Laboratory test: definition, biological specimens. Specific uses of biochemical tests and measure units METABOLIC PROFILES OF DIFFERENT ORGANS AND TISSUES Metabolic profiles: brain, heart muscle, liver, muscle, adipose tissue, kidney Tissue/organ interrelationships in response to different physiological (fed/fasting state; stress) and pathological (diabetes, ischemia) conditions. Liver as fuel provider for other tissues. Interrelationships between liver and skeletal muscle: Cori cycle and Alanine-Glucose Cycle. Role of hormones in the interactions between tissues and organs. DIABETES MELLITUS Carbohydrate properties and metabolism. Hormone regulation of glucose metabolism: insulin and counter-regulatory hormones. Ketone bodies formation and physiological role. Definition and classification of diabetes mellitus. Diagnostic criteria. Clinical presentation, history, pathogenesis and physiopathology of type 1 and type 2 diabetes. Laboratory tests in diagnosis and follow up of patients (monitoring the disease and therapy efficacy): fasting plasma glucose (FPG), urinary glucose, self-monitoring of blood glucose, oral glucose tolerance test (OGTT), insulin, C-peptide, glycated haemoglobin (HbA1c). Laboratory tests in acute complications: diagnosis of diabetic ketoacidosis (DKA) and hyperglycemic, hyperosmolar non ketotic syndrome (HONK) LIPIDS AND LIPOPROTEIN DISORDERS Lipids properties and metabolism: digestion, absorption, transport and storage. Lipoproteins: classification, composition, synthesis and metabolism. Role of Apoproteins. Lipoprotein receptors. Laboratory tests in diagnosis and monitoring therapy: measurement of triglycerides, cholesterol, HDL-cholesterol, LDL-cholesterol; lipoprotein electrophoresis. Expected values of lipids. CARDIOVASCULAR DISORDERS Dyslipoproteinemias: definition and classification. Atherosclerosis: plaque formation- role of LDL/HDL cholesterol. Non lipid risk factors associated with coronary artery disease (CAD).Patient's global risk and therapeutic goals of lowering LDL-cholesterol levels to prevent cardiovascular diseases. Biochemistry in physiological and pathological conditions of myocardium tissue: energy metabolism, enzymes in myocardial cells and contractile proteins in myocardial cells. Diagnostic criteria of Acute Myocardial infarction (AMI). Laboratory in the diagnosis and management of AMI: myoglobin; creatine kinase activity(CK) and determination of the isoenzyme CK-MB; troponins I and T.

For a fruitful study of the subject, and for an adequate understanding of the didactic materials are required notions of general and inorganic chemistry, biochemistry and molecular biology.

Clinical Chemistry (8th Edition): William Marshall Márta Lapsley Andrew Day; edited by Elsevier The Big Picture: Medical Biochemistry: Lee W. Janson, Marc E. Tischler; edited by Mc Graw Hill

mandatory attendance

The written test consists in the administration of a questionnaire of 45 questions (15 questions related to the General pathology-general Ptophysiology modules, 10 questions for the Clinical Pathology module, 10 questions for Clinical Biochemistry, 10 questions for the Immunology module.

The lessons are held face to face