Biomedical Laboratory techniques REPLICA B - ROMA AZIENDA S.CAMILLO FORLANINI

General principles and applications of clinical molecular biology. Brief history of molecular biology. Nucleotides and nucleic acids; genes, genomes and chromosomes; genetic code. Structure and function of DNA and its replication. Structure and function of RNA and its transcription. Structure and function of proteins and their translation. Purposes of molecular diagnostic tests. Basic methods in molecular biology. Nucleic acid extraction. PCR and real time PCR. DNA sequencing. Electrophoresis. Mutational search and genetic diseases, with application examples.

Capranico, Martegani, Musci, Raugei, Russo, Zambrano, Zappavigna. Biologia Molecolare. Edizioni Edises. Amaldi, Benedetti, Pesole, Plevani. Tecniche e metodi per la Biologia molecolare. Edizioni CEA (casa editrice ambrosiana)

Material uploaded on e-learning