Biomedical Laboratory techniques

During the third year, the student acquires the knowledge, skills and attitudes that satisfy the expectations required by the training path: autonomy, responsibility, management. At the end of the third year of training, the student must: • independently manage the pre-analytical, analytical and post-analytical phase • interpret the analytical data and proceed with any validation of the same • deliver the correct fulfillment of analytical procedures, the application of work protocols, the control and maintenance of laboratory instruments with professional technical autonomy • having acquired behavioral rules in the specific work context, so as to guarantee mastery of all the necessary skills and their immediate use in the work environment; • have acquired specific technical-diagnostic-professional skills in: CLINICAL PATHOLOGY Performing blood count tests with automatic analyzers Preparation of hematological preparations for microscopic examination for the determination of the leukocyte formula and reticulocytes with routine staining Optical microscopic observation of hematological preparations to identify normal elements Execution of typing techniques of blood groups (ABO, Rh), direct and indirect Coombs test, compatibility tests Performing assays in immunometry, radioimmunology, nephelometry, chromatography Performing the chemical / physical examination of the urine, with preparation of the preparations for the microscopic examination of the sediment and knowing the main elements of greatest clinical evidence Preparation of preparations for chemical-physical examination of the stool and the search for occult blood Reading and interpretation of electrophoretic traces of plasma proteins Performing the main coagulation tests (PT, PTT, antithrombin and fibrinogen) MICROBIOLOGY Preparation of preparations of various biological materials for observation under an optical microscope Execution of the main and suitable colors and identification of the main morphological characteristics of microorganisms Selection of the appropriate culture media according to the biological sample to be examined Execution of subcultures and transplants of bacterial jambs Execution of the various tests for the identification of the most frequently encountered clinical organisms Preparation of antibiograms and interpretation of the results Preparation of preparations for research of parasites, fungi and dermatophytes PATHOLOGICAL ANATOMY Acceptance and registration of cytological samples and anatomical pieces Preparation of cytological preparations Execution of routine and special cytological stains Preparation of histological preparations Execution of the microtome and cryostat cut Preparation of the slides with the sections obtained Execution of routine and special histological stains Execution of histochemical and immunohistochemical staining techniques Execution of techniques for the preparation of preparations for electron microscopy Management of the inclusions and slides archive Apply autoptic techniques Apply the conservation and disposal procedures for anatomical pieces MOLECULAR BIOLOGY Execution of manual techniques and in automation of extraction amplification in PCR End Point and Real Time PCR, hybridization, detection, genotyping and sequencing of nucleic acids

In order to be able to attend the internship in the analysis laboratories of the Umberto I polyclinic, students must comply with the prerequisites required for the year of the course.

The tutor provides the student with the teaching material and laboratory protocols where the internship takes place.

During the third year, students must attend the clinical analysis laboratories located within the Umberto I Polyclinic and La Sapienza University, according to the shifts assigned to them, in order to reach a frequency of 625 hours. During this time, students must work, in collaboration with the supervisor, on the topic of the future thesis.

The internship exam takes place in the presence of a commission made up of 4 tutors who followed the student during the practical training in the laboratories and the didactic director, the mark out of thirty is assigned through an overall evaluation of the internship period and with a final exam both oral than I practice.

During the third year, students must attend the clinical analysis laboratories located within the Umberto I Polyclinic and La Sapienza University, according to the shifts assigned to them, in order to reach a frequency of 625 hours. During this time, students must work, in collaboration with the supervisor, on the topic of the future thesis.

During the third year the acquisition of knowledge, skills and attitudes increases for the student to meet the expectations required by the training course: autonomy, responsibility, management. At the end of the third year of internship, the student must: • autonomously manage the pre-analytical, analytical and post-analytical phase • interpret the analytical data and proceed with its eventual validation • supply with professional technical autonomy the correct fulfillment of the analytical procedures, the application of the protocols work, control and maintenance of laboratory equipment • have acquired behavioral rules in the specific work context, so as to guarantee mastery of all the necessary ones skills and their immediate use in the workplace; • have acquired specific technical-diagnostic-professional skills in: CLINICAL PATHOLOGY Performing blood count tests with automatic analyzers Preparation of hematological preparations for microscopic examination for the determination of the leukocyte formula and reticulocytes with routine staining Optical microscope observation of hematological preparations to identify normal elements Performing blood group typing techniques (ABO, Rh), direct and indirect Coombs test, compatibility tests Execution of assays in immunometry, radioimmunology, nephelometry, chromatography Execution of the chemical / physical examination of the urine, with preparation of preparations for the microscopic examination of the sediment and knowing the main elements of greatest clinical feedback Preparation of preparations for chemical-physical examination of faeces and the search for occult blood Reading and interpretation of the electrophoretic traces of plasma proteins Carrying out the main coagulation tests (PT, PTT, antithrombin and fibrinogen) MICROBIOLOGY Preparation of preparations of various biological materials for observation under an optical microscope Execution of the main and suitable colors and identification of the main morphological characteristics of the microorganisms Selection of suitable culture media according to the biological sample to be examined Performing subcultures and transplants of bacterial stems Execution of the various identification tests of the microorganisms of the most frequent clinical finding Preparation of antibiograms and interpretation of results Preparation of preparations for research of parasites, fungi and dermatophytes PATHOLOGICAL ANATOMY Acceptance and registration of cytological samples and anatomical pieces Preparation of cytological preparations Performing routine and special cytological staining Preparation of histological preparations Performing the cut with the microtome and cryostat Preparation of the slides with the sections obtained Performing routine and special histological staining Performing histochemical and immunohistochemical staining techniques Execution of techniques for the preparation of preparations for electron microscopy Management of the archive of inclusions and slides Apply autopsy techniques Apply the procedures of conservation and disposal of anatomical pieces MOLECULAR BIOLOGY Execution of manual and automated techniques of extraction amplification in PCR End Point and Real Time PCR, hybridization, detection, genotyping and sequencing of nucleic acids

In order to be able to attend the internship at the analysis laboratories of the Umberto I polyclinic, students must comply with the prerequisites required for the year of the course.

The tutor provides the student with didactic material and laboratory protocols where the internship is carried out.

During the third year, students must attend the clinical analysis laboratories present within the Umberto I Polyclinic and La Sapienza University, according to the shifts assigned to them, in order to reach a frequency of 625 hours. During this time, students must work, in collaboration with the supervisor, on the topic of the future thesis.

Attendance is compulsory

The internship exam takes place in the presence of a commission made up of 4 tutors who followed the student during the practical training in the laboratories and the didactic director, the mark out of thirty is assigned through an overall evaluation of the internship period and with a final exam both oral than practical.

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During the third year, students must attend the clinical analysis laboratories present within the Umberto I Polyclinic and La Sapienza University, according to the shifts assigned to them, in order to reach a frequency of 625 hours. During this time, students must work, in collaboration with the supervisor, on the topic of the future thesis.