Pharmaceutical Biotechnology | Course catalogue

BIOCHEMICAL BIOTECHNOLOGIES

Course objectives

The course aims to provide the biochemical basis for understanding the development of technologies used in the research and production of proteins, enzymes and biomolecules useful for medicine, nutrition and industrial change. During the course, the potential applications of biochemistry and biotechnology in the industrial field will be illustrated, with reference to the pharmaceutical industry. Knowledge acquired Understanding of the methodologies used for the development and production on an industrial scale of proteins and enzymes. Knowledge of the general principles of protein engineering and advanced molecular biology techniques. Knowledge of the application potential of protein biotechnology in industrial, diagnostic and therapeutic fields, with reference to the pharmaceutical industry. Skills acquired Ability to use molecular and biochemical techniques for the purification, analysis and use of enzymes and biomolecules. Know how to design and produce recombinant proteins and their variants. Evaluate and choose the best technical strategies for the specific use. Communication and learning skills Gain autonomy in the development of experimental protocols and the choice of molecular biology and biochemical techniques for the expression and production of recombinant proteins and enzymes. Describe and critically relate the processes studied. Read scientific articles in the field of biochemistry-biotechnology and gain a critical understanding of their contents.

Channel 1

FABIO ALTIERI Lecturers' profile

Program - Frequency - Exams

Course program

Production of recombinant proteins. Design and implementation of protein variants. Cloning and mutagenesis methods, including recombination cloning methods (Gateway, TOPO, CRE-LOX etc.) and random mutagenesis Production of enzymes and proteins: downstream operations for recovery, separation of biomass from the culture medium, cell breakdown, analytical techniques for purification of biological macromolecules. Fusion proteins. Tags, types and uses. Green Fluorescent Protein. Purification of recombinant proteins with His-tag. Analytical methods for the characterisation of enzymes and proteins. Enzymes and proteins for industrial applications. Therapeutic proteins. Market for industrial enzymes and its characteristics. General information on enzymes. Mechanism of action, measurement of kinetic parameters, reversible and irreversible inhibitors. Effect of pH and temperature on enzymatic activity. Mechanisms regulating enzymatic activity. Modification of enzymatic characteristics: molecular evolution and techniques used (mutagenesis and recombination). Examples of protein engineering for biotechnology applications. Industrial application of enzymes: free and immobilized enzymes. Immobilization techniques. Stability and kinetics of immobilized enzymes. Bioreactors. Enzymes of industrial interest (lipase, protease, esterase) and examples of applications in industrial processes. Biotechnology and pharmaceutical industry. Applications of enzymes in the production of pharmaceuticals and biopharmaceuticals. Production of antibiotics and vitamins. Enzymes in the therapeutic (ERT ), analytical (biosensors and indicators) and diagnostic field.

Prerequisites

Basic knowledge of Biochemistry and Molecular Biology

Books

Metodologie biochimiche e biomolecolari Strumenti e tecniche per il laboratorio del nuovo millennio autore: Mauro Maccarrone (Zanichelli) Biocatalysts and enzyme technology, Buchholz et al., Wiley-VCH Biocatalysis, Bommarius & Riebel, Wiley-VCH Biotecnologia Molecolare Glick-Pasternak, Zanichelli Articoli scientifici presentati a lezione e altro materiale didattico fornito durante il corso e disponibile sul sito elearning. Selected scientific papers and educational material will be assigned during the course (web site elearning)

Frequency

Attendance is highly encouraged

Lesson mode

Lessons in class and exercises for 36 hours according to the schedule defined by the Board of the Study Course

SERENA RINALDO Lecturers' profile