THREE-DIMENSIONAL MODELING

Course objectives

Understand the main methodologies for determining protein structures. Learn the main methods used in proteomic analysis, the types of data generated, and their key limitations. Determine the thermodynamic stability of proteins and understand their mechanisms of folding. Mechanisms of aggregation and fibrillogenesis. Intermolecular interactions: binding affinity and association/dissociation rate constants. Learn the general principles of the design, heterologous production and mutagenesis of proteins. Acquire capacity of critical reading of scientific papers.

Channel 1
FRANCESCA CUTRUZZOLA' Lecturers' profile

Program - Frequency - Exams

Course program
Isolation of Protein-coding Genes Cloning: Classical and Recombinant Methods (GATEWAY/TOPO/CRE-LOX) Protein Expression: Vectors, Solubility, Tag Use, Cell-Free Expression Rational Mutagenesis: Classical and PCR Methods Random Mutagenesis and Guided Evolution TALEN, ZFN, and CRISPR/CAS Applied to Protein Engineering Examples of First- and Second-Generation Protein Drugs Examples of Applications in Biomedicine Discussion of Aspects Related to Specific Proteins and Nanoparticles (Articles - See Website)
Books
Mauro Maccarrone Metodologie biochimiche e biomolecolari Strumenti e tecniche per il laboratorio del nuovo millennio Selected literature reviews will be indicated during the course and on the e-learning platform.
  • Academic year2025/2026
  • CourseMedical Biotechnology
  • CurriculumBiomolecolare
  • Year1st year
  • Semester2nd semester
  • SSDBIO/11
  • CFU3