THREE-DIMENSIONAL MODELING

Course objectives

The module is dedicated to illustrate what are fungi, why they can be the agents of deterioration of cultural heritage and what are the strategies to prevent or cure their negative action. In addition, the course includes an introduction to molecular biology and in particular to DNA and PCR in the context of a broader reference to the strategies currently used for the recognition of these biological deterioration fungal species.. Specific skills A) Knowledge and understanding - Fungi - Fungi and biodeterioration of the cultural heritage - Strategies for prevention and treatment of fungal spoilage - 4. isolation strategies and recognition of spoilage fungi - 5. DNA and PCR - 6. DNA barcoding and molecular taxonomy B) Applying knowledge and understanding - how using specific terminology - Identify the main factors due to the bio-deterioration of cultural heritage - Identify the important activities for fungal bio-deterioration - Outline strategies for controlling the deteriorating fungi of cultural heritage - Recognize the deterioration fungi of cultural heritage through classical and molecular techniques C) Making judgements - Identification of new perspectives / development strategies for the protection of cultural heritage - Evaluation, interpretation and reprocessing of literature data in the field of bio-deterioration of cultural heritage D) Communication skills - Ability to illustrate the results of research and experimentation carried out in the context of the exercises - Ability to understand manuscripts in English and to indicate the salient features of the oral exam E) Learning skills - Learn the specific terminology of microbiologists - Logically connect the acquired knowledge in the field of bio-deterioration of cultural heritage - Identify the most relevant topics of the subjects dealt with - how consulting specialist databases (e.g. ncbi)

Channel 1
MARZIA BECCACCIOLI Lecturers' profile

Program - Frequency - Exams

Course program
1. Fungi 2. Fungi and biodeterioration of the cultural heritage 3. Strategies for prevention and treatment of fungal spoilage 4. Isolation strategies and recognition of spoilage fungi 5. DNA and PCR 6. DNA barcoding and molecular taxonomy 7. Practical exercises on molecular recognition of spoilage fungi LABORATORY SESSIONS (12 hrs). Isolation and recognition experiments using standard and molecular techniques of biodeteriogenic fungi from real material
Prerequisites
The teaching of Laboratory of Plant Materials in the triennial course is in the 2nd year, included among the mandatory courses. The knowledge that would be important to have acquired are those provided by the teaching of General Biology and Chemistry.
Books
PART 1: FUNGI One textbook at student’s choice between the followings: • Fondamenti di patologia vegetale, Editore: Pàtron, Edizione:2; A cura di:F. Favaron, F. Scala; Data di Pubblicazione:2017; EAN:9788855533829; ISBN:8855533827 • Biologia delle piante di Raven di Ray F. Evert, Susan E. Eichhorn, Editore: Zanichelli, Edizione: 7; Data di Pubblicazione: dicembre 2013; EAN: 9788808175045; ISBN: 8808175049 PART 2: DETECTION OF BIODETERIOGENOUS FUNGI One textbook at student’s choice between the followings: • Micologia Moderna. JW Deacon. Edagricole 2000. ISBN-10: 8820645521; ISBN-13: 978-8820645526 • Il gene X. Editore: Zanichelli; Anno edizione: 2012; EAN: 9788808159588
Teaching mode
The course includes lectures, themed seminars conducted by experts and laboratory sessions. Through the lectures, students learn the fundamental knowledge of the discipline. During the laboratory sessions of plant materials, the student learns individually to work in sterility, to work under an optical microscope, to prepare solutions with a known concentration, to assess visually the damage foreseen for a fungal deterioration, to isolate fungi from matrices of interest. , to withdraw in sterility fungi and extract nucleic acids, to use DNA to carry out an explorative PCR, to evaluate the result of an explorative PCR
Frequency
Lectures are not mandatory. At least 75% of lab sessions are mandatory.
Exam mode
The assessment consists of an oral interview. The oral exam consisting of at least 3 questions concerning the whole program, including lectures and exercises, is evaluated in 30ths. The minimum mark for passing the test is 18/30.
Bibliography
For news on textbooks and teaching materials see: https://elearning2.uniroma1.it
Lesson mode
Frontal lession (2CFU) and 1CFU of practise
  • Academic year2025/2026
  • CourseTechnologies for Conservation and Restoration of Cultural Heritage
  • CurriculumSingle curriculum
  • Year2nd year
  • Semester1st semester
  • SSDAGR/12
  • CFU3