Molecular dynamics in plant-microbe interactions: the study of plant-microbe interactions to develop biotechnological approaches for crop improvement

Course objectives

General outcome The main objective of this course is to provide the student knowledge on the molecular, cellular and evolutionary aspects of plant immunity, and on similarities and differences with the immune mechanisms of animals. The student will also acquire knowledge on the molecular basis of communication (recognition, elicitation, responses) between plants and microorganisms, both pathogenic and beneficial, and the mechanisms of control of the immune response that are the basis of pathogenicity and symbiosis. Finally, during the course the student will understand how the knowledge acquired in the study of plant-microorganism interactions is the foundation for the development of biotechnological approaches. The course also aims to provide students with the ability to use bibliographic resources and to clearly present complex scientific contents related to the aspects covered by the program. Specific outcomes A. Knowledge and understanding - Specific language and terminology. - The mechanisms that form the innate immune system of plants - Similarities and differences between the plant and animal immune systems. - The elements, processes and mechanisms of molecular and cellular regulation relevant in the interactions between plants and microbes (pathogenic and beneficial). - The dynamics of coevolution between plants and microbes. - The main methods of study adopted in these subjects. - The socio-economic problems related to issues in this specific field and object of classical and innovative biotechnological strategies. B. Applying knowledge and understanding - Ability to use specific terminology - Ability to outline the appropriate conceptual and methodological paths to address problems and questions in the field of plant-microbe interactions. - Ability to use bibliographic resources, software and biological resources available through the Web to address and interpret specific problems related to the aspects object of this course. C. Making judgements - Critical judgment skills, through the study of reviews and scientific articles on key aspects and through in-depth collective discussions; - Ability to evaluate correctness and scientific rigor through analysis and collective discussion of the experimental and methodological part of recent high quality scientific articles. D. Communication skills - Acquisition of adequate skills and tools useful for communication, through the use of graphic and formal languages, with particular regard to scientific language, through discussions and seminars that are an integral part of the oral exam. E. Learning skills The student will possess: - the ability to set up an independent and flexible study method, which allows to conduct personal research and analysis and to continue effectively the advancement of knowledge. - the ability to identify problematic, unresolved and innovative aspects in biological issues - the ability to find and use cognitive tools for the continuous updating of knowledge - the ability to compare oneself for the progress, consolidation and improvement of one's own knowledge. Results Possession of the fundamental contents of the specific discipline, and the ability to master the procedures and methods of their own investigation, also to orientate and operate in the field of applied sciences.

Channel 1
GIULIA DE LORENZO Lecturers' profile

Program - Frequency - Exams

Course program
Below is the entire program of the course, which is carried out in co-teaching. - General concepts. Pathogenic microorganisms and symbionts. Arabidopsis thaliana, Nicotiana tabacum (tobacco), Hordeum vulgare (barley) and Triticum aestivum (wheat) as model systems for studying plant-pathogen interaction. Penetration mechanisms. Degradation of cell walls, enzymes and their inhibitors. - Introduction to the immune system. Defense mechanisms of plants at the site of infection: preformed and inducible, structural and biochemical mechanisms. Systemic defense responses. Response to pests and herbivores. - Genetic control in plant-microorganism interactions; gene-for-gene resistance; molecular recognition. Virulence molecules/effectors. R proteins: NBS-LRR leucine-rich repeat (LRR) modular proteins in response to pathogens. ETI: “Effector-triggered immunity”. - Danger signals for the plant (or elicitors). General elicitors: Microbe-Associated (MAMP) and Damage-Associated (DAMP) Molecular Patterns. The cell wall in the signaling of tissue damage. PTI: “Pattern-Triggered Immunity”. Pattern recognition receptors (PRR) and their co-receptors for recognition of pathogens and symbionts, Dynamics of elicitor perception complexes. - Generalities on effectors. Bacterial effectors: examples. Effectors of fungi and oomycetes: examples. - PTI and ETI as interdependent mechanisms. - The LRR protein structure in plant and animal immune response and hormone responses. Structure-function relationships in receptors for the recognition of MAMP, DAMP and resistance proteins. Chimeric receptors. Conservation, similarities and differences between the animal and plant immune systems. - Transduction cascades in defence: role of phosphorylation/dephosphorylation, ubiquitination and other post-translational modifications. Interaction between transduction pathways involved in defense and development. Hormonal interactions in the defense response. - Systemic resistance: Acquired systemic resistance, Induced systemic resistance, Systemic response to injury. - Plant microbiota interactions: effects on growth and response to pathogens. Beneficial microorganisms with particular attention to those used in agriculture as biofertilizers, biostimulants or bioprotectors. PGPR/PGPF: Plant growth promoting Rhizobacteria and Fungi.
Prerequisites
Basic knowledge of plant physiology, molecular biology, biological chemistry.
Books
Agrios (Fifth Edition) Plant Pathology, Academic Press. Chapters 1-6. Biochemistry and Molecular Biology of Plants (Second Edition). John Wiley & Sons, 2015 - Capitolo 21. Educational support material in https://elearning2.uniroma1.it/course/view.php?id=621
Frequency
Attendance is not mandatory but strongly recommended due to the complexity and variety of topics covered.
Exam mode
The evaluation will be based on the interest and activity demonstrated by the student during the lectures and seminars. It will be assessed whether the student understood the basic principles and concepts, was able to explore questions, analyze and solve scientific problems, and engage in active learning. The evaluation is expressed in thirtieths (minimum grade 18/30, maximum mark 30/30 with honors)
Lesson mode
Learning methods includes lectures and classroom presentations of specific topics with discussion, using telematic supports. Through the lectures students learn the fundamental knowledge of the discipline.
DANIELA PONTIGGIA Lecturers' profile

Program - Frequency - Exams

Course program
Detailed Program Below is the entire program of the course, which is carried out in co-teaching. - General concepts. Pathogenic microorganisms and symbionts. Arabidopsis thaliana, Nicotiana tabacum (tobacco), Hordeum vulgare (barley) and Triticum aestivum (wheat) as model systems for studying plant-pathogen interaction. Penetration mechanisms. Degradation of cell walls, enzymes and their inhibitors. - Introduction to the immune system. Defense mechanisms of plants at the site of infection: preformed and inducible, structural and biochemical mechanisms. Systemic defense responses. Response to pests and herbivores. - Genetic control in plant-microorganism interactions; gene-for-gene resistance; molecular recognition. Virulence molecules/effectors. R proteins: NBS-LRR leucine-rich repeat (LRR) modular proteins in response to pathogens. ETI: “Effector-triggered immunity”. - Danger signals for the plant (or elicitors). General elicitors: Microbe-Associated (MAMP) and Damage-Associated (DAMP) Molecular Patterns. The cell wall in the signaling of tissue damage. PTI: “Pattern-Triggered Immunity”. Pattern recognition receptors (PRR) and their co-receptors for recognition of pathogens and symbionts, Dynamics of elicitor perception complexes. - Generalities on effectors. Bacterial effectors: examples. Effectors of fungi and oomycetes: examples. - PTI and ETI as interdependent mechanisms. - The LRR protein structure in plant and animal immune response and hormone responses. Structure-function relationships in receptors for the recognition of MAMP, DAMP and resistance proteins. Chimeric receptors. Conservation, similarities and differences between the animal and plant immune systems. - Transduction cascades in defence: role of phosphorylation/dephosphorylation, ubiquitination and other post-translational modifications. Interaction between transduction pathways involved in defense and development. Hormonal interactions in the defense response. - Systemic resistance: Acquired systemic resistance, Induced systemic resistance, Systemic response to injury. - Plant microbiota interactions: effects on growth and response to pathogens. Beneficial microorganisms with particular attention to those used in agriculture as biofertilizers, biostimulants or bioprotectors. PGPR/PGPF: Plant growth promoting Rhizobacteria and Fungi.
Prerequisites
Basic knowledge of plant physiology, molecular biology, biological chemistry.
Books
Educational support material in https://elearning2.uniroma1.it/course/view.php?id=621 Agrios (Fifth Edition) Plant Pathology, Academic Press. Chapters 1-6. Biochemistry and Molecular Biology of Plants (Second Edition). John Wiley & Sons, 2015 - Capitolo 21. Reverberi et al. Patologia vegetale molecolare, Piccin-Nuova Libraria.
Frequency
Attendance is not mandatory but strongly recommended due to the complexity and variety of topics covered
Exam mode
The evaluation will be based on the interest and activity demonstrated by the student during the lectures and seminars. It will be assessed whether the student understood the basic principles and concepts, was able to explore questions, analyze and solve scientific problems, and engage in active learning. The evaluation is expressed in thirtieths (minimum grade 18/30, maximum mark 30/30 with honors)
Lesson mode
Learning methods includes lectures and classroom presentations of specific topics with discussion, using telematic supports. Through the lectures students learn the fundamental knowledge of the discipline. The frequency of teaching classes is not mandatory, but is strongly encouraged.
  • Lesson code10600071
  • Academic year2025/2026
  • CourseCell biology and technology
  • CurriculumSingle curriculum
  • Year1st year
  • Semester2nd semester
  • SSDBIO/04
  • CFU6