Biotechnology and Genomic for Industry and Environment

The teaching of Advanced Analytical Methodologies has the main objective of providing fundamental knowledge and basic principles of separative techniques and spectroscopic methods. As regards the separative techniques, in addition to the theoretical principles, the operating principles and methods of application, in real systems, of the main separative techniques, mass spectrometry and coupling techniques are illustrated. Furthermore, the principles and main fields of application of atomic and molecular spectroscopy will be addressed: atomic absorption by flame and graphite furnace; plasma spectroscopy; UV-Vis spectrophotometry and notes of X-ray fluorescence. The main objective of the course is therefore to make the students able to acquire, after the study of the main characteristics of separation and spectroscopic techniques, critical skills, in relation to the analytical problem, in the choice of a suitable sample analysis technique real, together with aspects related to sampling, sample preparation and data processing. The lessons related to separative techniques, starting from the theory of extraction and chromatography, deepen the study and description of the different types of separative techniques (gas chromatography, liquid chromatography and capillary electrophoresis) coupled with different detectors, including the mass spectrometer. As for atomic spectroscopy, the general theory principles will be faced up to the description of atomic absorption by flame and graphite furnace; plasma spectroscopy. For both analytical techniques specific applications will be addressed on the analysis of different classes of compounds of biotechnological and environmental interest, etc. The knowledge acquired in this teaching, will be a point of reference and starting point, but also a means to acquire successive and interdisciplinary skills Students who have passed the exam will be able to know and understand (acquired knowledge) - the main techniques, separative and spectroscopic and their evolution - the main working methods, with practical examples of application in different fields - the most recent literature in this area - aspects related to the current applications of separation techniques to biotechnological, environmental, industrial, food, toxicological, metabolomic, proteomic and clinical problems Students who have passed the exam will be able to (skills and skills acquired): - studying in deep critically and autonomously the purposes of separative methodologies and the relative applicative potentiality. - understand the connection with other cultural areas of the CdS -develop the ability to communicate what has been learned, through oral interviews and tests. - develop self-study using accessible sources of updating.