Biomedical Laboratory techniques REPLICA B - ROMA AZIENDA S.CAMILLO FORLANINI

Main diagnostic methodologies in immunological and microbiological field including mononuclear cell separation from peripheral blood and functional assays, diagnostic application of monoclonal antibodies (direct and indirect immunofluorescence, flow cytometry, microscopy, western blot, ELISA), methodologies for diagnosis of immunodeficiencies, autoimmune diseases and allergies, molecular methodologies (nucleic acid extraction, RT-PCR and real time PCR) culture techniques and identification of micro-organisms. Students will be able to critically apply their theoretical knowledges, learn laboratory safety rules and improve their operational skills.

No specific prerequisites are required. However, basic notions of biochemistry, molecular and cellular biology, anatomy and immunology are required for the understanding of the course contents and the achievement of the learning objectives.

teaching material proposed by the appointed Professor.

mandatory

comprehension on the main methodologies and laboratory safety

Seminar and practical activities for learning the following laboratory techniques: Nucleic acid extraction Gene amplification Sanger sequencing NGS sequencing Immunogenetics Oncology and oncohematology genetic

Basic concepts of genetic diseases and methods of cytogenetic and molecular analysis