Biomedical Laboratory techniques REPLICA B - ROMA AZIENDA S.CAMILLO FORLANINI

Part I: THE DIAGNOSTICS LABORATORY - Sampling, collection and storage of biological materials - Analytical variability and measurement errors - Control of the methods used in a diagnostic laboratory (accuracy and precision) - Quality control in the diagnostic laboratory - Biological variability and reference values - Diagnostic efficacy of a laboratory test (sensitivity, specificity, predictive value, ROC curves) Part II: BASIS OF THE MAIN ANALYTICAL TECHNIQUES AND THEIR APPLICATION - Absorption spectrophotometry: Lambert-Beer law, instrumentation, analytical methods for the determination of the main parameters of Clinical Chemistry - Methods of separation in homogeneous phase: chromatographic techniques - Immunochemical techniques: radioisotopic (R.I.A. and I.R.M.A.); alternative (chemiluminescence, immunofluorescence, immunoenzymatic techniques) - Techniques for the determination of specific proteins: turbidimetry, nephelometry, immunonefelometry - Principles of electrophoretic techniques: agarose gel electrophoresis, polyacrylamide, capillary; immunoblotting and immunoprecipitation - Methods for the determination of enzymatic activities: examples of enzymes of clinical interest - Haematological cytology in automation: operating principles and techniques applied to flow cytofluorimeter

In order to understand the teaching content and to achieve the learning objectives, at the beginning of the didactic activities foreseen by the didactic module the student must possess the indispensable basic knowledge of biochemistry and molecular biology

- MEDICINA DI LABORATORIO, A cura di Giorgio Federici, Mc Graw-Hill Editore, 3a edizione 2008, ISBN 978-88-386-3941-8 - BIOCHIMICA CLINICA E MEDICINA DI LABORATORIO, di Marcello Ciaccio e Giuseppe Lippi, EdiSeS Editore, ISBN 978-88-7959-958-0 - BIOCHIMICA CLINICA vol 1 e 2, di Luigi Spandrio, Piccin Editore - MEDICINA DI LABORATORIO, Laposata, Piccin Editore, ISBN 978-88-299-2109-6

The teacher delivers lectures with traditional methods, blended methods or remote methods, depending on the existing provisions, with audiovisual aids and scheduling of lessons as reported on GOMP Aule/Orari system, published on the website of the Degree Course

Compulsory attendance

The exam consists of an oral exam that deals with one or more topics of the individual modules in detail. The knowledge related to each module contributes together with the overall assessment. To pass the exam the student must obtain a grade of not less than 18/30 in each module. The student should demonstrate that he has acquired sufficient knowledge of the topics of clinical biochemistry and a basic knowledge of the topics of management of the diagnostic laboratory, and he should be able to indicate the main diagnostic applications of each method. To achieve a score of 30/30 cum laude, the student must instead demonstrate that he has acquired excellent knowledge of all the topics covered during the course, being able to link them in a logical and consistent way, treating the laboratory techniques with focus on their quality and efficiency , and placing their use in relation to the required diagnostic investigations. The frequency of lessons is also taken into consideration for the evaluation. Exams are held at the end of the course.

Additional teaching material presented during the lessons will be provided to the students either in electronic format or as paper material. It will also be recommended useful material for any further study.

The teacher delivers lectures with traditional methods, blended methods or remote methods, depending on the existing provisions, with audiovisual aids and scheduling of lessons as reported on GOMP Aule/Orari system, published on the website of the Degree Course