THREE-DIMENSIONAL MODELING

Course objectives

General skills The course includes two modules: one of Histology and one of Embryology. The main objective of the course is to lead the student to know the structural and ultrastructural characteristics of the tissues, correlating the structure to function, and to understand the morphogenic processes through which a body is built during embryogenesis, with a comparative vision to different animal models (mainly vertebrates). General skills – Module of Histology The course is focussed on the study of the morpho-functional characteristics of the tissue, and on the general mechanisms regulating their omeostasis. The objective of the course is to lead the student to understand the structural and ultra-structural organization of cells and tissues and to correlate structure to function. Moreover, to know the methodological and experimental aspects of histology and how to identify and describe the histological structure of a microscope slide. The course requires established knowledge of cell biology, as well as general notions of inorganic and organic chemistry. The course includes lectures integrated with two laboratory sessions, dedicated to the observation and recognition of histological sections of the different tissues. General skills – Module of Embryology The course is focused on the study of embryology and the different phases of embryogenesis and on the gametogenesis and fertilization processes. The main aim of the course is to acquire the basic knowledge of the various phases of embryogenesis with a comparative view on different animal models (mainly vertebrates), of the maturation of gametes and the mechanisms of fertilization. The course requires established knowledge of cell biology. The course includes lectures integrated with one laboratory session, dedicated to the observation and recognition of histological sections of vertebrate embryos at various developmental stages. Specific skills – Module of Histology A) Knowledge and understanding - Knowledge of the structural and ultrastructural organization of the tissues - Knowledge and understanding the correlation between structure and function - Knowledge and understanding the general mechanisms regulating tissue homeostasis B) Applying knowledge and understanding - learning how to use the appropriate terminology - learning the methods used in histology - learning how to recognize the structure of a tissue at Light Microscope and its location within the organ. C) Autonomy of judgment - acquiring critical judgment skills, through the study of methodological and experimental aspects of histology, as well as their present and future value in the biomedical applications and physiopathology. D) Communication skills - Learning how to present the acquired knowledge in an interview, using proper scientific language and knowing how to integrate, discuss and analyze in a critical way what has been learned. E) Learning skills - learning the appropriate terminology - learning to connect and integrate the acquired knowledge in a logical way - learning to identify the most relevant topics and their potential applicability to current problems. Specific skills – Module of Embryology A) Knowledge and understanding - Knowledge of the main events of embryogenesis - Knowledge and understanding of the various stages of embryo development with a comparative vision - Knowledge and understanding of the germ line formation and the gametes maturation. B) Applying knowledge and understanding - learning how to use the appropriate terminology - learning how to recognize the various phases of embryogenesis - learning how to recognize the various embryos and the main tissues and organs in development in images and diagrams C) Autonomy of judgment - acquiring critical judgment skills, through the historical study of the embryological knowledge - evaluation of the acquired knowledge through a written test D) Communication skills - Learning how to present the acquired knowledge in a written test using proper scientific language and knowing how to integrate, discuss and analyze in a critical way what has been learned E) Learning skills - learning the appropriate terminology - learning to connect and integrate the acquired knowledge in a logical way - learning to identify the most relevant topics and their potential applicability to current problems.

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GIUSEPPE LUPO Lecturers' profile

Program - Frequency - Exams

Course program
Historical perspective: from embryology to developmental biology. Early developmental steps: cleavage, gastrulation and organogenesis. Egg classification depending on the yolk content. Different types of cleavage and the influence of yolk on cleavage. Gastrulation: morphogenetic movements and germ layer formation. Direct and indirect development. Main animal model systems for the study of developmental biology. Reproduction and sexuality. Gametogenesis: main steps and general features. Spermatogenesis: sperm cell differentiation and maturation. Testicle organization in mammals. Oogenesis: egg cell differentiation and maturation. Different types of meiotic arrest during oogenesis. Ovary organization in mammals. Ormonal control of gametogenesis in humans. General features of fertilization. Chemiotaxis, sperm cell activation, acrosomal reaction, sperm-egg interactions. Polispermy inhibition: fast and slow inhibition. Cortical reaction. Egg activation and the initiation of embryogenesis. Main steps in sea urchin and mammalian fertlization. Main features in the embryogenesis (cleavage, gastrulation and neurulation) of model organisms: sea urchin, ascidian, zebrafish, Xenopus, chick, mouse and human development. Fate maps at blastula and early gastrula stages. Axes of embryonic polarity. Embryonic annexes. Organogenesis: main derivatives of ectoderm, mesoderm and endoderm germ layers. Ectoderm: nervous system and neural crest cells. Mesoderm: chordomesoderm, paraxial mesoderm, intermediate mesoderm and lateral plate mesoderm. Germ celldevelopment and sex determination.
Prerequisites
The course requires the knowledge acquired through the Cell Biology course held during the first term of the first year of the Biotechnology course and a basic knowledge of general Chemistry.
Books
Recommended textbook: Menegola, Bonfanti, Colombo, Del Giacco, Manuale di Biologia dello Sviluppo Animale, EdiSES (2019). Additional material is made available by the teacher on the e-learning2 platform: https://elearning2.uniroma1.it/course/view.php?id=1932
Frequency
Lectures are not mandatory.
Exam mode
The final exam test aims to verify the level of knowledge and in-depth analysis of the topics covered and the critical analysis developed by the student. The test is written and based on images and schemes that must be recognized and commented by the students.The evaluation is expressed in thirtieths (minimum grade 18/30, maximum mark 30/30 cum laude). The exam includes an oral examination with the faculty staff in charge of the Istology module and the final evaluation mark reflects the average of the marks in each the two modules.
  • Academic year2025/2026
  • CourseBiotechnologies
  • CurriculumSingle curriculum
  • Year1st year
  • Semester2nd semester
  • SSDBIO/06
  • CFU3