Methods in Behavioral Neuroscience

Course objectives

Learning objectives The topic of this course is the neurobiological study of animal behaviour in a laboratory setting. The main aim of the course is to provide students with advanced knowledge about current multidisciplinary methods for the study of animal behaviour and its biological basis, in normal conditions and in preclinical models of human pathologies. Specific attention will be paid to bioethical and legislative issues regarding the use of animal models in biomedical research. The course involves lectures, classroom/laboratory exercises and the participation to a collaborative group assignement. Knowledge and understanding - knowledge and understanding of the main preclinical animal models of human pathologies of the central nervous system - knowledge and understanding of the current methods for the study the biological basis of behaviour - knowledge and understanding of the rationale behind the use of animal models and related bioethical issues - knowledge and understanding of the main paradigms for the study of animal behaviour in a laboratory setting - knowledge and understanding of the advantages and limitations of the different methods used in behavioural neuroscience - knowledge and understanding of primary scientific literature in the behavioural neuroscience field Applying knowledge and understanding - ability to use the specific terminology of behavioral neuroscience - ability to interpret the research design in a behavioral neuroscience experiment - ability to identify, compare and evaluate the methodological approaches available for answering experimental questions in the behavioural neuroscience field Making judgements - ability to discuss critically a scientific research article in the behavioural neuroscience field Communication skills - ability to communicate effectively the acquired knowledge in writing - ability to communicate effectively the acquired knowledge orally Lifelong learning skills - upon completion of the course students should be able to understand and evaluate independently primary research literature in the behavioural neuroscience field - upon completion of the course students should be able to plan independently a simple experimental design to test hypotheses in behavioural neuroscience

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ARIANNA RINALDI Lecturers' profile

Program - Frequency - Exams

Course program
The topic of the course "Methods in Behavioral Neuroscience" is the neurobiological study of animal behaviour in a laboratory setting, with a primary focus on mouse models. This course will explore multidisciplinary approaches for investigating the biological correlates of behaviour in normal and pathological conditions. Specific attention will be paid to bioethical and legislative issues regarding the use of animal models in biomedical research. Introduction to the main animal models used in biomedical research. Animal models of neurological and psychiatric disorders. Methodological aspects. Experimental design. ARRIVE Guidelines. Ethics and legislation in animal research. General physical condition of the animal. Primary neurological-behavioral screening. Genetically modified animals, CRISPR/Cas9, conditional systems. Strains. Parkinson's disease. Huntington's disease. Amyotrophic Lateral Sclerosis: models of motor and non-motor symptoms. Assessment of motor functions. Ataxias. Rotarod test. Alzheimer's disease: animal models for the study of memory. Morris Water maze, Radial maze, Cross Maze, Barnes Maze, Y-maze. Schizophrenia: sensorimotor integration, startle and prepulse inhibition. Animal models of stress. Tests for anxiety, depression and fear: Elevated-plus maze, Elevated zero maze, dark-light box, sucrose preference test, Porsolt test, tail suspension test, fear conditioning. Study of structure-function relationship and recognition memory. Object recognition test. Three-chambers test. Environmental enrichment. Techniques for the study of dendritic spines. Methods for the study of pain. Stereotaxic techniques and behavioral pharmacology. Viral vectors for the manipulation of neural activity. Chemogenetics and Optogenetics. Electrophysiological recordings. Markers of neuronal activity in vitro and in vivo. Statistical analysis and data presentation. Practical sessions: histology, poster preparation, behavior analysis, ARRIVE Guidelines and experimental design.
Prerequisites
This course requires a good knowledge of the basic elements of molecular and cellular biology acquired during the bachelor's degree, which is considered verified through the admission process for the master's degree in Neurobiology. The course requires a good knowledge of the English language and the ability to engage with original material written in English (textbooks, articles from international scientific literature).
Books
Reference books, lecture notes and supplementary readings are available on the course page on Sapienza's elearning website: https://elearning.uniroma1.it (Scienze matematiche, fisiche e naturali / Biologia / Lauree Magistrali / Neurobiologia / Metodi di Neuroscienze Comportamentali AA. 2025-2026).
Teaching mode
The course is organized in lectures and classroom/laboratory exercises. The total duration of the course is 48 hours, with two lessons per week (2 hours/lesson)
Frequency
Attendance is not mandatory, but strongly recommended. The collaborative group assignment can only be taken by students who have attended at least 60% of the lectures.
Exam mode
The assessment includes a collaborative group assignment, a written exam and an optional oral exam. The collaborative group assignment aims to develop the students' ability to elaborate a topic independently, collaborate and communicate effectively what they have learned. The written and oral exams aim to verify the level of knowledge and in-depth analysis of the topics covered in the course and the critical thinking skills developed by the student. The final grade will be expressed out of 30 (minimum grade 18/30, maximum grade 30/30 cum laude). - The collaborative group assignment consists of the preparation and oral presentation in poster format of a group report on a topic related to the syllabus, which will be assigned after the first half of the course. The maximum mark attributed to the group assignment is 4 points, which will be added to those of the written/oral exam. The mid-term assignment is not mandatory, but strongly recommended and can only be taken by students who have attended at least 60% of the lectures. - The written exam includes 7 open-ended questions. The maximum time allowed to complete the exam is 120 minutes. The maximum mark attributed to each question is 4 points. - The oral exam can be requested by the student to improve the grade obtained in the written exam and can lead to an increase (or decrease) of up to 3 points, compared to the sum of the written exam score and the collaborative group assignment score. The minimum score to be admitted to the oral exam is 25/30 (sum of the written exam score and the mid-term assignment score). The oral exam is mandatory for those who do not take the collaborative group assignment and can lead to an increase (or decrease) of up to 4 points compared to the written exam score. Students who have not taken the collaborative group assignment must have achieved at least 18/30 in the written exam to be eligible for the oral exam. To achieve a mark of 30/30 cum laude, the student must demonstrate excellent knowledge of all the topics covered during the course and critical thinking.
Bibliography
Lecture notes and supplementary readings (in english) will be available on the course page on Sapienza's elearning website: https://elearning.uniroma1.it (Scienze matematiche, fisiche e naturali / Biologia / Lauree Magistrali / Neurobiologia /Metodi di Neuroscienze Comportamentali AA. 2023-2024)
Lesson mode
The course is organized in lectures and classroom/laboratory exercises. The total duration of the course is 48 hours, with two lessons per week (2 hours/lesson).
  • Lesson code10592906
  • Academic year2025/2026
  • CourseNeurobiology
  • CurriculumSingle curriculum
  • Year1st year
  • Semester2nd semester
  • SSDM-PSI/02
  • CFU6