Methods in Behavioral Neuroscience

Course objectives

Learning objectives The topic of this course is the neurobiological study of animal behaviour in a laboratory setting. The main aim of the course is to provide students with advanced knowledge about current multidisciplinary methods for the study of animal behaviour and its biological basis, in normal conditions and in preclinical models of human pathologies. Specific attention will be paid to bioethical and legislative issues regarding the use of animal models in biomedical research. The course involves lectures, classroom/laboratory exercises and the participation to a collaborative group assignement. Knowledge and understanding - knowledge and understanding of the main preclinical animal models of human pathologies of the central nervous system - knowledge and understanding of the current methods for the study the biological basis of behaviour - knowledge and understanding of the rationale behind the use of animal models and related bioethical issues - knowledge and understanding of the main paradigms for the study of animal behaviour in a laboratory setting - knowledge and understanding of the advantages and limitations of the different methods used in behavioural neuroscience - knowledge and understanding of primary scientific literature in the behavioural neuroscience field Applying knowledge and understanding - ability to use the specific terminology of behavioral neuroscience - ability to interpret the research design in a behavioral neuroscience experiment - ability to identify, compare and evaluate the methodological approaches available for answering experimental questions in the behavioural neuroscience field Making judgements - ability to discuss critically a scientific research article in the behavioural neuroscience field Communication skills - ability to communicate effectively the acquired knowledge in writing - ability to communicate effectively the acquired knowledge orally Lifelong learning skills - upon completion of the course students should be able to understand and evaluate independently primary research literature in the behavioural neuroscience field - upon completion of the course students should be able to plan independently a simple experimental design to test hypotheses in behavioural neuroscience

Channel 1
ARIANNA RINALDI Lecturers' profile

Program - Frequency - Exams

Course program
The topic of the course "Methods in Behavioral Neuroscience" is the neurobiological study of animal behaviour in a laboratory setting. This course will explore multidisciplinary approaches for investigating the biological correlates of behaviour in normal and pathological conditions. Specific attention will be paid to bioethical and legislative issues regarding the use of animal models in biomedical research. Introduction to techniques used to study animal behavior in biomedical research. Introduction to the most important animal models in biomedical research. Law and Ethics in research with animals. Animal models of neurological and psychiatric disorders: Methodological aspects. Genetically modified animals, CRISPR/Cas9, strains. Assessment of general physical condition of the animal. Primary neurological-behavioral screening. Measurement of motor functions. models of motor and non-motor symptoms. Animal models of Huntington's disease, Amyotrophic Lateral Sclerosis. Ataxia. Rotarod test. Alzheimer's disease: animal models for the study of memory. Morris Water maze, Cross Maze, Fear Conditioning. Techniques for studying the structure-function relationship. Object recognition test. Behavioral pharmacology and stereotaxic techniques. Electrophysiological recordings: place cells and grid cells. Viral-mediated gene expression. Schizophrenia: sensorimotor integration, startle and prepulse inhibition. Techniques for the visualization of neural activity. Animal models of stress, anxiety and depression. Models of Autism, social behavior. Chemogenetics and Optogenetics. Environmental enrichment. Techniques to study dendritic spines. Statistical analysis and data presentation: articles and oral communications. Environmental enrichment. ARRIVE Guidelines Laboratory practice (Istology, Excel, Poster preparation, behavioural analysis).
Prerequisites
This course requires good knowledge of basic molecular and cellular biology, as acquired during the bachelor's degree. Moreover this module requires good knowledge of english and the ability to read and understand original scientific research articles and book chapters written in english.
Books
Reference books, lecture notes and supplementary readings are available on the course page on Sapienza's elearning website: https://elearning.uniroma1.it (Scienze matematiche, fisiche e naturali / Biologia / Lauree Magistrali / Neurobiologia / Metodi di Neuroscienze Comportamentali AA. 2023-2024).
Teaching mode
The course is organized in lectures and classroom/laboratory exercises. The total duration of the course is 48 hours, with two lessons per week (2 hours/lesson)
Frequency
Attendance is not mandatory, but strongly recommended
Exam mode
The assessment includes a collaborative group assignment, a written exam and an optional oral exam. The collaborative group assignment aims to develop the students' ability to elaborate a topic independently, collaborate and communicate effectively what they have learned. The written and oral exams aim to verify the level of knowledge and in-depth analysis of the topics covered in the course and the critical thinking skills developed by the student. The final evaluation will be expressed in 30 (minimum grade 18/30, maximum grade 30/30 with honors). The collaborative group assignment consists of a written report and an oral presentation in the form of a scientific poster on a relevant topic, that will be assigned during the course. The maximum mark attributed to the group assignment is 4 points, which will be added to those of the written/oral exam. The written test includes 7 open-ended questions. The maximum time allowed to complete the test is 120 minutes. The maximum mark attributed to each question is 4 points. The minimum mark to be admitted to the oral exam is 18/30. The oral exam can be requested by the student to improve the mark obtained in the written test and can lead to an increase (or reduction) of up to 3 points. The minimum score to be admitted to the oral is 25/30. The oral test is mandatory for those who do not take the collaborative group assignment. To achieve a mark of 30/30 cum laude, the student must demonstrate excellent knowledge of all the topics covered during the course and critical thinking.
Bibliography
Lecture notes and supplementary readings (in english) will be available on the course page on Sapienza's elearning website: https://elearning.uniroma1.it (Scienze matematiche, fisiche e naturali / Biologia / Lauree Magistrali / Neurobiologia /Metodi di Neuroscienze Comportamentali AA. 2023-2024)
Lesson mode
The course is organized in lectures and classroom/laboratory exercises. The total duration of the course is 48 hours, with two lessons per week (2 hours/lesson)
  • Lesson code10592906
  • Academic year2024/2025
  • CourseNeurobiology
  • CurriculumSingle curriculum
  • Year1st year
  • Semester2nd semester
  • SSDM-PSI/02
  • CFU6
  • Subject areaAttività formative affini o integrative