Educational objectives The use of instruments with increasingly high magnetic fields, the continuous progress in the fields of computer science and electronics, a more in-depth examination of the basic theory, the use of increasingly sophisticated pulse sequences ad hoc developed to obtain specific information about the studied systems allowed the application of Nuclear Magnetic Resonance spectroscopy to diverse areas concerning the study of complex systems, expanding the field of use from the initial chemical-physical and organic field to every possible chemical sector. The aim of the course is to provide theoretical and practical knowledge of the physical bases of Nuclear Magnetic Resonance and specifically illustrate the applications to the most modern developments in the field of Analytical Chemistry. The primary objective of the course is to provide students with the necessary tools to qualitatively and quantitatively analyze complex and real systems such as natural extracts and industrial products. The main knowledge acquired by the students will be:
Basic NMR theory
Methods of execution and processing of modern NMR experiments (1D and 2D sequences)
Knowledge and application of NMR parameters in general and specifically in the field of Analytical Chemistry.
q-NMR approaches.
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Educational objectives The course is structured in order to provide an overview of the basic techniques listed in the program, illustrating their theoretical concepts and operating principles, as well as possible problems. The main objectives include:
- to address students to the correct identification of the most suitable analytical technique;
- to provide the knowledge to be able to optimize the instrumental conditions, focusing on qualitative and quantitative analysis;
- to improve the student's ability to adapt the instrumental method to the different analytical problem;
- - to improve the student's ability to critically process the results of instrumental analysis.
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Educational objectives The educational objectives that this course intends to achieve are:
- Learning of problems related to sampling and sample preparation techniques prior to the instrumental chemical analysis.
- In-depth knowledge of the main extraction and instrumental separation techniques (chromatographic, electrophoretic, in the supercritical phase and in fractionation in the flow field).
- Knowledge of the parameters necessary for the validation of an analytical method.
At the end of the course, organized in lectures and in parallel to practical laboratory experiences, students will be able to:
- Development, improvement, and validation of an analytical method to perform trace analysis of analytes in different real matrices, planning the extraction, separation and chromatographic detection procedures
- Chromatogram Interpretation
- Performing a qualitative and quantitative analysis with the statistical treatment of the data
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Educational objectives The lectures are aimed to provide to the students an educational path starting from the basic concepts of mass spectrometry up to its last developments and applications in the fields of analytical chemistry and biomolecules study.
At the end of the course, the students have to demonstrate the knowledge of the theory and basic principles of mass spectrometry, as well as of the various ionization techniques and mass analyzers. Moreover, the students should have understood the potential of the coupling between liquid or gas chromatography and mass spectrometry, as well as tandem mass spectrometry, in particular concerning complex mixtures and compounds at trace levels. Furthermore, the students have to be able to extrapolate and describe the main data and information obtainable from a mass spectrum.
Concerning the application of the knowledge, in case of a real problem, the students should possess the capability of select both the most suitable instrumentation and acquisition modes for the analysis of biological, environmental, and food samples. The capability of arguing the choice of possible analytical strategies is another important objective.
Finally, self-study capability should be proven by gaining further insight into specific course topics with the aid of the scientific literature.
The aims of the course are described in detail according to the five Dublin descriptors.
Dublin Descriptor 1 – Knowledge and understanding
At the end of the course, the students have learned the basic theoretical principles of mass spectrometry, as well as of the various ionization techniques and the main mass analyzers. The students have to know the theory of tandem mass spectrometry and understand the possibilities concerning both qualitative and quantitative determination. They have to understand the potential of the coupling of tandem mass spectrometry with separative techniques or the possibility of very fast “in situ” analyses. They have to recognize between information obtainable from low- and high-resolution mass spectrometry and to understand the concept of mass accuracy.
Dublin Descriptor 2 - Applying knowledge and understanding
The students have to acquire the capability of facing a complex analytical problem with the aid of mass spectrometry and tandem mass spectrometry, for example for applications in environmental or food analysis. It is important also to take into account the related European law concerning the maximum allowable limits of certain substances depending on the limit of detection of the technique and its various acquisition modes. The students have to be able to select the most suitable ionization technique depending on target analytes and sample origin, also suggesting a possible coupling between mass spectrometry and a separation technique.
Dublin Descriptor 3 - Making judgments
The students have to develop the capability of critical evaluation concerning an analytical or general research problem, which requires the application of mass spectrometry, by connecting the knowledge acquired during the whole study course. This capability is developed by the aid of examples from the scientific literature, with particular emphasis on complex mixtures of compounds present at trace level and/or structurally unknown, and biomolecules (e.g. proteins and peptides). It is also important the capability of justifying the choice of the analytical strategy.
Dublin Descriptor 4 – Communication skills
The students have to be able to write in a report or verbally communicate the acquired knowledge, in a concise, coherent, and well-focalized way, also by the aid of graphic informatic tools, to be understandable by both specialized and non-specialized audience.
Dublin Descriptor 5 – Learning skills
At the end of the course, the students should have developed suitable tools to stimulate detailed studies and links between different topics. They should possess the skills to independently refer to the scientific literature related to mass spectrometry to deepen both some theoretical aspects and, most of all, application aspects. By referring to the scientific literature, the students have to be able to obtain the information to solve new problems, as well as to get the fundamental tools useful for their professional activity.
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Educational objectives Chemistry and Characterization Nanostructured Materials aims to provide fundamental knowledge and basic principles for the study of materials and nanomaterials, starting from their specific surface and bulk characteristics, to their characterization methods, to new materials and nanomaterials properties and performances, of interest for the Analytical Chemistry. The aim of this course is to highlight the correlations between chemical structures and looking at the most recent applications of nanomaterials. The lectures are
developed starting from the description of the general characteristics of materials and nanomaterials chemistry to characterization methods, their preparation and functionalization, to obtain materials and nanomaterials for applicative studies. The knowledge acquired in this course constitutes a framework of reference for subsequent competences.
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Educational objectives - Learn the problems associated with sampling and extraction techniques preliminary to liquid and gas chromatographic analysis.
- Understand and define the main parameters necessary for the validation of an analytical method.
At the end of the course, organized in lectures followed by practical laboratory exercises, numerical exercises in the classroom and flipped teaching, students will be able to:
- experimentally develop an analytical method
- validate an analytical method according to the main validation protocols
- carry out a quantitative LC or GC analysis.
- perform the statistical treatment of the results.
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Educational objectives The aim of the course is to instruct the students on the structural identification of complex organic compounds via their spectrometrical properties. The first part of the course involves the theoretical knowledge on the 1H NMR, 13C NMR, EI-MS and IR spectrometriy with a particular attention on the relationships between spectral morphology and molecular structure. The second part of the course is dedicated to the comparative analysis of a set of spectra in order to identify the structure of different classes of complex organic compounds.
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