Nanotechnology Engineering

1) Classical Electromagnetism; Complex refractive index; classical oscillator model of Lorentz; dispersion of the complex index; Kramers-Kronig relations; Snell and Fresnel laws; diopters; lenses; spherical mirrors; physical optics: interference and diffraction; limit of resolution of the optical microscope; brief description of the quantum approach to the polarizability. - Lab Session 1 (Dipartimento SBAI - Prof. F.Michelotti): Laboratory practice with Snell laws; measurement of the refractive index of a liquid; practice with lenses and mirrors. 2) Energetic levels in organic molecules; Electronic levels and simmetry of the orbitals; Morse potential; vibrational, rotational and spin levels; singlet and triplet states; Linear absorption; Spontaneous and stimulated emission; Selection rules; Franck-Condon principle; Absorption spectroscopy; Absorption spectra of most diffused endogenous and exogenous chromophores; Jablonski diagram: internal conversion, inter-system crossing, quenching; Forster energy transfer; fluorescence and phosphorescence; Stationary and time-resolved luminescence spectroscopy; Emission spectra of most diffused endogenous and exogenous chromophores; Solvatochromism; Photobleaching; Advanced microscopic techniques: FLIM, FRET, FRAP, TIRFM; Nonlinear Optics; Fourier description of the 2nd and 3rd order dielectric susceptivities; Second harmonic generation; Kerr effect; Nonlinear absorption: two-photon absorption and excited state absorption; Spontaneous and stimulated Raman emission; Nonlinear optical microscopies: Raman, SRS, CARS, TPA, SHG; Super-resolution microscopies: STED, PALM, STORM; DNA microarrays; Label free techniques; Sanger sequencing; Sequencing by means of laser techniques; ELISA test ; Labelling techniques making use of fluorescent markers; Surface chemical functionalization techniques. - Lab Session 2 (Dipartimento SBAI - Dr. A. Occhicone): Absorption spectroscopy in alcoholic and water solution of selected organic molecules. - Lab Session 3 (Dipartimento SBAI - Dr. A. Occhicone): CW fluorescence spectroscopy in alcoholic and water solution of selected organic molecules. - Lab Session 4 (Dipartimento SBAI - Dr. A. Occhicone):): Use of a wide-field fluorescence microscope to study biological tissues and cell cultures. - Lab Session 5 (OPBG – Ospedale Pediatrico Bambino Gesù - Prof. F. Michelotti, Dott.ssa S. Petrini): Use of a confocal/FRET/FRAP fluorescence microscope to study biological tissues and cell cultures 3) Properties of surface plasmon polaritons on uniform thin metal films(SPP), Dispersion of the SPP, characteirstics of the plasmonic modes, SPP excitation by measn of ATR, Detection platforms based on SPP for biological applications. - Lab session 6 (Dipartimento SBAI- Dr. Occhicone): Measurement of the SPP resonance in ATR regime ; Resonance shift due to the injection of several different liquids with varying refractive index; photonic crystal sensors.

Good basic knowledges in General Physics, including time dependent electric and magnetic fields, in Chemistry and in Mathematical analysis.

1) Notes distributed during the lectures. 2) E. Hecth, Optics, Pearson Educational Limited, (Harlow), 2014. 3) J.Lakowicz, Principles of Fluorescence Spectroscopy, 3rd Edition, Springer (Berlin), 2007.

All communications concerning the Biophotonics Laboratory Course will be handled by means of the Google Classromm available at: https://classroom.google.com/c/MjI2NjcyMzA2NzEw?cjc=2uxncsq (Code: 2uxncsq) The lectures will held in the rooms indicated in the timetable published on the Website of the Nanotechnology Engineering Council: https://corsidilaurea.uniroma1.it/it/corso/2021/30429/home https://www.ing.uniroma1.it/sites/default/files/orari_lezioni/MNAR3Ib.pdf The lectures will also be broadcasted online by means of either Zoom or Google Meet. The links to the the Zoom or Google Meet rooms will be published on the Classroom of the course.

The lectures will be complemented by a series of laboratory sessions during which the student will deal with experiments in optics, spectroscopy. Moreover there will be a series of visits in some selected research labs to show the operation of fluorescence, confocal and more advanced microscopes.

The evaluation will be based on procedures and criteria that are published on the personal website of the teacher at: https://www.sbai.uniroma1.it/~francesco.michelotti/AA21-21_Lezioni%20di%20Biophotonics%20Laboratory.pdf

All communications concerning the Biophotonics Laboratory Course will be handled by means of the Google Classromm available at: https://classroom.google.com/c/ODA3NTQ4MDQxNTQ1?cjc=shjqwukn The lectures will held in the rooms indicated in the timetable published on the Website of the Nanotechnology Engineering Council: https://docs.google.com/spreadsheets/u/0/d/e/2PACX-1vQEkUNQPLk4jZrN1Hs6oN1TFOM-2ZukjF2I13EPtkfWceRtDJDabpbunK0GPzxcjCLFsvxn1WnC60MC/pubhtml?pli=1#gid=830010379